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KMID : 0974620000180020041
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Bulletin of Dongnam Health University
2000 Volume.18 No. 2 p.41 ~ p.54
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Studies on Antibiotics Resistance of the Klebsiella pneumoniae Strains isolated from Patients.
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池寧鎭/Ji, Young Jin
白亨基/洪性魯/曺道鉉/Bak, Hyung Ki/Hong, Sung No/Cho, Do Hyun
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Abstract
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One hundred strains of Klebsiella pneumoniae were isolated from feces of dysenteric patients who were resistant to ampicillin but sensitive to nalidixic acid. Sixteen out of the 100 isolates were resistant to chloramphenicol and cephalothin, 16 to tetracycline, 13 to streptomycin, 8 to kanamycin and 2 to gentamycin. The distribution of resistance to different combination of eight antimicrobioal agents was as follows. 68% were resistant to 2 antibiotics, 15% to the combination of three antibiotics, 12% to the mixture of 4 chemicals, 6% to 5 mixture, 3% to 6 antibiotics and 2% to the combination of 7 antibiotics.
Electorphoresis patterns of the plasmid DNA of K. pneumoniae were investigated. 98KP8, 98KP9 and 98KP10 had a plasmid of 45.8Md, 44.1Md and 47.8Md, respectively. 98KP1 had four plasmids where their molecular weight larged from 29.2Md to 81.2Md. 98KP2 also had four plasmids and the molecular weight ranged from 4.9Md to 94.lMd.
EcoR I restriction fragments of the K. pneumoniae R plasmid DNA were sperated by elctrophoresis.
The EcoR I restriction fragment analysis showed that there is no correlation between the number of plasmid and the diversity of the fragment. Moreover we could not observe the similarity of the fragment among the strains which have the same resistance against the same combination of antibiotics.
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